Subsequently competent cells were prepared and transformed. After large-scale preparation of plasmid DNA, recombined adenoviruses Ad5F35-IL24 were generated with homologous recombination by cotransfection of 293 cells with a couple plasmids.
制备感受态细胞,进行转化,大量制备质粒DNA;之后用双质粒共转染293细胞,产生重组腺病毒Ad5F35-IL24。
参考来源 - 腺病毒介导IL·2,447,543篇论文数据，部分数据来源于NoteExpress
Objective to explore the co operative inhibitory effect of antisense VEGF gene and endostatin gene transfection on tumor angiogenesis, tumor growth and metastasis of lung cancer.
目的探讨反义vegf基因和内皮抑素基因联合转染在抑制肿瘤血管生成和肿瘤生长转移中的作用。
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
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