The purified DNA can be used directly in subsequent enzymatic reactions such as labeling, sequencing, cloning, and ligation, as well as for PCR analysis.
纯化的DNA可直接用于后续的应用，如标记、测序、克隆、连接和PCR分析。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
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