Clone formation ability was determined by clony forming assay. Cell cycle and apoptosis were tested by flow cytometry.

通过流式细胞术检测细胞的细胞周期分布和凋亡水平；

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MethodsA clone formation assay was carried out to determine the clonogenicity and regeneration ability of cells.

方法采用有限稀释法，检测细胞单克隆形成的数量及大小；

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