To determine the proliferative effect of mechanical stretching on osteoblasts, cell cycle analysis was carried out by flow cytometry.
应用流式细胞技术研究机械拉伸应变对成骨细胞增殖的影响,探讨此类细胞在力学生理背景下的生长行为。
MTT assay was applied for cell proliferation detection, while Flow Cytometry (FCM) for cell cycle analysis.
采用MTT法检测细胞增殖活性;流式细胞仪(FCM)检测细胞周期的变化。
Cells were exposed to taxol at different concentrations for 6, 12, 24, 48 and 72h after which cell cycle analysis was performed with flow cytometer.
细胞暴露于不同浓度紫杉醇,分别于6、12、24、48、72小时收集标本、作流式细胞术细胞周期分析。
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