...培养和生物学特性研究 干细胞(placenta-derived mesenchymal stem cells,PMSCs)和兔骨髓间充质干细胞(bone marrow-derived MSCs,BMSCs)体外分离培养、增殖,对其生物学性状进行比较观察。
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Results (1)Mononuclear cell collected by using density centrifugation was detected by flow cytometer, phenotypes showed CD29, CD44 surface antigens of culture cells was positive, but CD34, CD45 surface antigens was negative, which indicated these cells were human Bone marrow-derived MSCs.
结果(1)经密度梯度离心法分离贴壁培养所得到的细胞,通过流式细胞仪检测的结果为:细胞表面标志物CD29、CD44为阳性,CD34、CD45为阴性,符合骨髓间充质干细胞特征。
参考来源 - 人骨髓间充质干细胞成骨分化特异性miRNA的筛选·2,447,543篇论文数据,部分数据来源于NoteExpress
Human umbilical cord-derived and human placenta-derived MSCs are easily available, which have more widespread application prospect than bone marrow-derived MSCs.
人脐带和胎盘来源的MSC取材容易,较骨髓间充质干细胞有更广泛的应用前景。
Conclusion: The evident difference is not found in biological characteristics and ability of differentiation into neuron-like cells between rat fetal blood and bone marrow-derived MSCs.
结论:大鼠胎血和骨髓msc的细胞形态、生物学特性无明显差别;两者诱导分化为神经元样细胞的能力无显著差异。
Objective: to compare the growth characteristics and differentiation into neuron-like cells of mesenchymal stem cells (MSCs) derived from rat fetal blood with MNCs from rat bone marrow in vitro.
目的:比较大鼠胎血和骨髓中间充质干细胞(MSC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同。
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