Results: Bait plasmids pGBKT7-IRE1N(1-465)and pGBKT7-IRE1C(468-977) were successfully constructed and the expression of fusion-proteins were correct. Tests for autonomous activation and toxicity were negative.
结果:成功构建诱饵质粒pGBKT7-IRE1N及pGBKT7-IRE1C,并在酵母中正确表达融合蛋白,自激活检测及毒性检测均为阴性。
参考来源 - 酵母双杂交筛选IRE1结合蛋白及其在肝细胞凋亡中的作用研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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