Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce Lactuca sativa l.
以GUS基因作为报告基因，利用农杆菌真空渗透法，采取正交试验设计对莴苣LactucasativaL。
It was concluded that using the nopaline type of Agrobacterium (e. g. C58), 5min vacuum infiltration, and using the plants with just several blooming flowers would give the best results.
其中以开花初期的植株，利用胭脂碱型农杆菌介导(如c58)，真空渗入5分钟的处理方法转化效率最高。
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