First, the crystallization thermodynamic data and other basic physicochemical data of solution, such as viscosity, density of 7-aca solution and dissociation constants, were measured by experiments.

本文首先实验测定了7 - ACA结晶热力学数据和粘度、密度、电离平衡常数等必要的基本物性数据。

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The genomic DNA was extracted from the selective cultures and the GL-7-ACA acylase gene was directly amplified by PCR technique.

从土壤的选择性培养液中提取微生物的DNA，通过PCR的方法直接扩增成功得到GL-7-ACA酰化酶基因。

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The results of joint action experiments showed that, except of the binary mixture of tetracycline and 7-aca had antagonistic effect, the other binary mixtures showed simple additive effect.

联合毒性实验结果显示除四环素和7 - ACA的二元混合物为拮抗作用外，其余二元混合物均为简单相加作用。

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