Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5 ' - UTR)启动基因表达的活性。
The sequencing of highly conserved 5' UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays.
HGV高度保守区5＇UTR的分子克隆与核苷酸序列的测定对开展HGV感染的基因诊断具有重要意义。
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