用纯化的抗原包被 微孔板，制成固相抗原，往包被单抗的微孔中依次加入黑色素瘤抗体（melanoma Ab），再与HRP标记的抗原结合，形成抗原-抗体-抗原复合物，经过彻底洗涤后加底物TMB显色。
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黑色素瘤相关抗原抗体 Anti-MAGE ; hematopoieticcells,tissuemacrophages.CD ; MAGE
目的：制作PPO的单克隆抗体并研究其在恶性黑色素瘤中的表达。
OBJECTIVE: To prepare the monoclonal antibody of PPO and study the expression in malignant melanoma.
黑色素瘤抗独特型抗体疫苗能模拟肿瘤相关抗原，激发机体的抗肿瘤免疫应答，已在许多动物实验和临床试验中得到证实。
It has been demonstrated that anti-idiotypic antibody vaccines against melanoma can mimic tumor-associated antigens and activate anti-tumor immune responses in many animal models and clinical trials.
Debs及其同事发现该抗体停止了小鼠胸腺癌、肺癌、结肠癌与黑色素瘤的继发性肿瘤生长。
Debs and his colleagues found that the antibody stopped the growth of secondary tumours from breast, lung, colon and melanoma cancers in mice.
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