结论：在较为适当的培养条件下，利用生物反应器可使工程细胞的培养达到高密度、高表达。
Conclusion: Under adequate culture conditions, high density and high expression culture of the recombinant cell line could be realized by using bioreactor.
高海水浓度培养条件下的HW—1的细胞生长没有密度依赖性，而低海水浓度(10%或更低)培养条件下的细胞生长具有一定的密度依赖性。
HW-1 cells from high seawater-containing medium grew independently of cell-density, while those from low-seawater media (10% or less) showed density-dependent growth.
目的：在应用生物反应器进行动物细胞大规模培养时，过程的在线分析和调控技术对于高细胞密度和高产品得率的获得是非常重要的。
Aim: On-line analysis and control are very important for the optimization and achievement of high cell concentration and product yield in animal cell culture.
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