近年来许多实验证实存在于骨髓中的间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)可向多种造血以外的组织迁移、定居,并分化为相应的组织细胞如骨组织、软骨组织、心肌细胞、神经细胞等[4...
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普通的间充质干细胞有多种来源(包括骨髓),但产量原没有脂肪组织中得到的多。
Normal mesenchymal cells can be isolated from various sources, including bone marrow, but the yield is not nearly as great as what the researchers derived from fat tissue.
由于脂肪组织与骨髓同为中胚层起源的组织,那么能否从脂肪组织中获得间充质干细胞,而且也具有多分化潜能呢?
Because both fat tissue and bone marrow are tissues originated from mesoderm, so whether MSCs could be obtained from fat tissue as well, which also have multi - lineage differentiation potential?
结论:经比较羟乙基淀粉沉淀法明显优于其他分离方法,在人骨髓间充质干细胞的培养过程中建议使用此种物理沉降方法分离骨髓有核细胞。
This physical sedimentation method is a very good method that can be used during the culture of MSCs to separate nucleated cells in bone marrow.
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