结果:成功地培养出小鼠的颅神经嵴细胞,其形态类似成纤维样细胞。
RESULTS: Cranial neural crest cells were cultured successfully. The shape of the cells were fibroblast-like.
方法:采用胰酶消化法分离小鼠胚胎第8 . 5天的颅神经管,从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞。
METHODS: cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells.
本文从牙齿发育信号的启动、颅神经嵴细胞在牙齿形态发生中的作用以及上皮-间充质的交互式作用三个方面简述牙齿发育的调控机制。
The regulation mechanism during tooth development, which includes the initiation of tooth developmental signal, the role of neural crest cells in tooth morphogenesis, and the interaction between dent.
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