方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
目的探讨氟化物对体外培养乳鼠颅盖骨细胞的生长、细胞周期和凋亡的影响。
Objective to investigate the effects of fluoride on growth, cell cycle and apoptosis in cultured calvarial cell from Suckling mice.
选用摘除双侧卵巢的山羊颅盖骨，体外分离培养成骨细胞，并对分离的成骨细胞做多方面生物学特性鉴定。
Osteoblasts were isolated from the frontal parietal bone of ovariectomized goat and were confirmed by testing for multiple biological features.
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