结果：确定两化合物与牛血清蛋白的猝灭机理主要为静态猝灭和非辐射能量转移。
RESULTS The quenching mechanism of them were suggested as static quenching and nonradiative energy transferring.
实验结果表明，茶碱与BSA形成基态复合物导致BSA内源荧光猝灭，猝灭机理主要为静态猝灭和非辐射能量转移。
The experimental results show that the fluorescence quenching of BSA by theophylline is due to the formation of theophylline-BSA complex through both static quenching and nonradiative energy transfer.
依据非辐射能量转移机理，测定了不同温度下溴甲酚紫与血清白蛋白相互结合时，其给体-受体间距离和能量转移效率。
According to the non-radiation energy transfer theory, the distances and energy transfer efficiencies between dye and protein at various temperatures were obtained.
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