1)采用Ⅳ型胶原酶、链霉蛋白酶E(PronaseE)灌注消化，Nycodenz密度梯度离心法得到SD大鼠肝脏非实质细胞(nonparenchymal cells，NPC)，再以选择性贴壁法纯化KC。
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利用形态学观察、记录细胞增殖状况,以及免疫荧光染色技术初步分析肝脏非实质细胞(nonparenchymalcell,NPC)的生长对于AHPC活化、增殖和分化的影响。另外,尝试了AHPC克隆的传代培养。
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Methods: We applied in site perfusion to isolate Spraqae-Dawley rat, obtained liver non-parenchymal cells(NPCs) with diversity centrifugal force(CF), utilized discontinuous density gradient and selectively adherence to isolate KCs, they were deposited in -80℃.
方法：原位灌注消化Spraqae-Dawley大鼠肝脏，应用不同离心力获取肝脏非实质细胞，不连续密度梯度离心、选择性贴壁分离肝脏枯否氏细胞，培养、-80℃冻存。
参考来源 - 枯否氏细胞NF·2,447,543篇论文数据，部分数据来源于NoteExpress
目的采用离心淘洗技术分离纯化大鼠肝非实质细胞亚群。
Objective In order to isolate and purify nonparenchymal cells subgroups from rat liver, centrifugal elutriation technique was applied.
由于这一屏障是由毛细血管壁构成，并非实质细胞，因此，脑组织的渗透性特征也不同于其他组织。
Because the capillary wall rather than the parenchymal cell forms the barrier, the brain's permeability characteristics differ from those of other tissues.
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