杂交时采用限制性显示（restriction display，RD）技术标记样 品，可囊括登革病毒的全基因组信息，实现对同一样品进行 大量序列检测和核酸分析的目的。
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结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
结论采用限制性差异显示技术制备DNA标记物，不仅操作简便、快捷、提高工作效益，且大大降低费用，更适合实验室实际需要。
Conclusion DNA marker can be produced for laboratory use with RD-PCR technology, which is a simple, efficient and inexpensive method for experiment.
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