结果证明pEN179-shRNA可以明显减轻PRRSV引起的特异性细胞病变;在间接免疫荧光分析(indirect immunofluorescent assay,IFA)和Western blot检测中,pEN179-shRNA处理细胞的PRRSV N蛋白阳性细胞及N蛋...
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结果证明pEN179-shRNA可以明显减轻PRRSV引起的特异性细胞病变；在间接免疫荧光分析(indirectimmunofluorescentassay，IFA)和Westernblot检测中，pEN179-shRNA处理细胞的PRRSVN蛋白阳性细胞及N蛋白表...
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在间接免疫荧光分析 indirect immunofluorescent assay
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
方法：采用间接免疫荧光标记，流式细胞术分析。
Methods Indirect immune fluorescent labeling and flow cytometry were employed.
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