基因工程的建立离不开各种核酸酶剪刀,锌指核酸酶(zinc finger nuclease)就是又一把锋利的刀:。锌指核酸酶能对双链DNA的特定位点进行剪切,因此在基因敲除、基因加工等领域具有重要的应用价值。
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他还补充说锌指核酸酶可能对一些基因具有不可预知的作用。
He adds that the zinc finger nucleases could have unpredictable effects on several genes.
他们在使烟草细胞接触锌指核酸酶之外,也添加了可增强抗除草剂特性的基因片段。
When they exposed tobacco cells to the zinc finger nucleases, they also added a version of that gene that enhances herbicide resistance.
锌指核酸酶被设计识别基因组特定DNA序列,之后对其进行酶切,进而删除或替换这段序列。
Zinc-finger nucleases are enzymes that can be designed to find specific DNA sequences in the genome and cut them out, deleting those sequences or swapping one gene for another.
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