The presence of the biotinylated allele-matched probe is then detected by the QCM via the binding to streptavidin-peroxide horseradish (SA-HRP), which catalyzes the oxidative precipitation of 3, 3-diaminobenzidine (DAB) by H2O2 on the electrode and provides an amplified frequency response.
生物素化的检测探针继而与链酶亲和素化的辣根过氧化物(SA-HRP)绑定,辣根过氧化物催化过氧化氢氧化底物中的3, 3–二氨基联苯胺(DAB)在电极表面生成不溶性沉淀物,使压电频率响应显著增大。
参考来源 - 基因检测和点突变识别的DNA探针及适配体生物传感新方法研究·2,447,543篇论文数据,部分数据来源于NoteExpress
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