六、 操作规程 A、cDNA第一条链的合成(First-strand cDNA Synthesis): 1、 使用前每个组份轻轻混匀,然后2000rpm离心20s。
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该研究的下一步是设计更多的新的转运RNA,使得更多的非常规氨基酸能够被携带参与蛋白链的合成。
The next step in this direction is to design more new tRNAs that can incorporate more unnatural amino acids into protein chains.
由于在侧链的合成中,有手性碳原子发生了构型翻转,因此我们合成了一个相应的多利培南光学异构体,以利于质量控制。
Due to the configuration inversions of chiral carbon in the synthesis of side chain, we synthesized the corresponding optical isomer of doripenem for quality control.
现在的科技可以随意合成冗长的DNA链,这意味着我们已经能够复制病毒的基因组,而细菌基因组的复制也指日可待。
The ability to synthesise huge screeds of DNA at will means the genomes of viruses can be replicated already, and replicating those of bacteria is not far off.
So you made somehow an anti-sense polynucleotide to the insulin gene or some fraction of the insulin gene.
这样你就合成了,胰岛素基因的反义多聚核苷酸链,或者说胰岛素基因的片段
If somehow you could take your anti-sense molecules that you've made and you could get them into cells, then by this process of hybridization they would naturally form a pair like this.
如果你能得到合成的反义链,并能使它进入细胞,那么通过杂交,它们就能自然形成这样的一对
Well, that anti-sense strand is shown here as the red and the cell is naturally making the blue or sense strand of messenger RNA for a particular protein.
好的,反义链在这里以红色表示,细胞自身会造出合成某种,特定的蛋白质的蓝色RNA正义链
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