图1诱导培养后1周,普通光学显微镜下细胞聚集成团,形态变的不规则,呈铺路石样排列。
Figure 1 After 1 week of induced culturing, cells aggregated, morphology became irregular and aligned like gravels (Inverted phase contrast microscope).
结果纯化后的颌下腺腺泡细胞呈铺路石样形态,原代至第5代培养基及细胞内均可检测到淀粉酶表达;
Result Cobblestone-like submandibular gland acinar cells were found after purification, and amylase was successfully detected both in culture medium and cells of passage 0-5 respectively.
结果:小型香猪角质形成细胞呈“铺路石”样生长,可连续传代,实验结束时成功地将角质形成细胞传至第13代。
RESULTS: The keratinocytes of mini fragrant pigs grew like pave road stone, which can be subcultured serially. The keratinocytes were passed to 13 passages till the experiment was completed.
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