用紫外线诱变哈茨木霉野生型菌株,经含多菌灵PDA培养基耐药性筛选,获得了耐药性菌株。
Auxotrophic mutants of Trichoderma harzianum tolerant to carbendazim were obtained by UV-light mutagenesis and carbendazim induction on PDA medium.
接种的寄主组织中菌体生长能力检测发现,突变体的繁殖速度慢于野生型,但最终其菌体数量也能达到野生型菌株的最大值。
The inserted mutants grew more slowly than the wild-type strains, but their population could finally reach the maximum of the wild-type strains in inoculated leaf tissues of Hami-melon.
细胞试验表明重组菌对细胞的黏附力和相对侵袭力均有不同程度的降低,而相对侵袭力与野生型菌株具有显著性差异(P<0.05);
The recombinant strain also had lower adhesiveness(P>0.05)and significantly lower relative invasiveness to the HeLa cells than its wild type strain(P<0.05).
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