目的:构建弓形虫棒状体蛋白(ROP2 )基因重组质粒并在大肠杆菌中表达,用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的研究联合运用狂犬病毒糖蛋白基因重组腺病毒与同一抗原核酸疫苗对小鼠的免疫效果。
Objective To investigate the immune response of mice primed by DNA particle bombing and boosted by immunization with replication-defective adenoviral recombinant bearing rabies glycoprotein gene.
分别以重组蛋白疫苗、商品疫苗和PBS免疫仔猪,优化检测免疫抗体水平的间接ELISA条件,通过比较三个免疫组的抗体水平来评价表达蛋白作为免疫抗原的有效性。
Piglets of each groups were vaccinated respectively with recombinant protein vaccine, commodity vaccine and PBS as contrast. The serum IgG anti-protein of each groups were detected by indirect ELISA.
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