大容量人天然噬菌体单链抗体库的构建(论著) 关键词] 单链抗体(scFv)；噬菌体抗体库；重叠延伸拼接 [gap=1077]Key words] Single-chain antibody (scFv); Phage display library; Overlapping PCR
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用带有VH3′端、连接肽和VL5′端序列的引物修饰VL得VL′后,与VH进行重叠延伸拼接(splicing by overlap extension,SOE),连接成VH-Linker-VL(ScFv),并大量扩增.
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方法：设计合成抗KDR单抗(mAb)YcomlD3VH、VL引物，通过重叠延伸拼接(splicing overlap extensive，SOE)PCR，在VH和VL基因间引入柔性短肽(Gly4Ser)3，构建抗KDR scFv基因并进行序列分析...
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人趋化因子受体CCR5胞外片段的融合表达与纯化 行融合表达与纯化。方法分别扩增人趋化因子受体CCR5的胞外N-端部分与ECL-2部分相应的编码序列,通过重叠延伸拼接(Splicing byoverlapping extension,SOE)PCR实现两片段DNA的拼接后(命名为CCR5-N-E2)克隆入质粒pBlueScript M13中
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重叠延伸拼接法 splicing by overlap extension ; splicing by overlapping extension ; SOE
采用重叠延伸拼接法 splicing by overlapping extension ; splicing by overlap extention
用重叠延伸拼接 splicing by overlap extension ; SOE
利用重叠延伸拼接 splicing by overlap extension ; SOE
用重叠延伸拼接法 SOE ; splicing by overlap extention ; splicing by overlapping extension
计成重叠延伸拼接 splicing by overlap extension ; SOE
为重叠延伸拼接法 splicing by overlap extension
称为重叠延伸拼接法 SOE ; splicingby overlap extension
进行重叠延伸拼接 splicing by overlap ex-tension
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