...SLSEP)检测脑功能；采用缺口末端标记法(Terminal Deoxynucleotidyl Tranferase Mediated dUTP Bioton Nick End Labeling，TUNEL)检测额皮质和海马CA1区凋亡神经元数量及电镜观察海马CA1区神经元凋亡形态学改变。
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凋亡原位检测采用缺口末端标记法。
The apoptosis in situ was measured by the nicking end labeling.
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