...保存8h后，复灌期心脏作功量（rate-pressure product，RPP）变化情况，采用原位末端标记（TdT-mediated dUTP nick end labeling，TUNEL）染色法检测心肌细胞凋亡和免疫组织化学方法检测Fas和FasL蛋白表达情况。
基于4个网页-相关网页
采用原位末端标记法 TdT-mediated biotinylated-dUTP nick end labeling ; Terminal deoxynucleotidyl Transferase-mediated dUTP NickEnd Labeling ; TUNEL ; terminaldeoxynucleotidly transferase mediated labelling
方法：采用原位末端标记法。
方法采用原位切口末端标记方法检测细胞凋亡，平均每100个细胞内的阳性细胞数为凋亡指数(AI)。
Method Apoptosis was determined by the method of in situ Nick end labeling. Apoptotic index (AI) means the Numbers of apoptotic cells per 100 cells.
分别采用免疫组化方法和原位末端标记法检测垂体前叶细胞增殖核抗原的表达及凋亡的变化情况。
The changes of the proliferating nuclear antigen expression and apoptosis of anterior pituitary cells were detected with immunohistochemistry and in situ end labeling method.
应用推荐