...保存8h后，复灌期心脏作功量（rate-pressure product，RPP）变化情况，采用原位末端标记（TdT-mediated dUTP nick end labeling，TUNEL）染色法检测心肌细胞凋亡和免疫组织化学方法检测Fas和FasL蛋白表达情况。
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采用原位末端标记法 TdT-mediated biotinylated-dUTP nick end labeling ; Terminal deoxynucleotidyl Transferase-mediated dUTP NickEnd Labeling ; TUNEL ; terminaldeoxynucleotidly transferase mediated labelling
方法：采用原位末端标记法。
方法采用核固红结晶紫染色方法，原位末端标记法及形态计量方法定量分析了被动吸烟致金黄地鼠神经管上皮细胞凋亡的变化。
Methods By using the nuclear fast red-crystal violet staining method and situ TUNEL technology, the quantitative changes of apoptosis in neural epithelium in gold hamster were analyzed.
凋亡原位检测采用缺口末端标记法。
The apoptosis in situ was measured by the nicking end labeling.
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