目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
结果成功构建了以GFP为报告基因的酿酒酵母载体，并在酵母中得到表达。
Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully.
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