酵母双杂交系统是将待研究的两种蛋白质分别克隆(融合)到酵母表达质粒的转录激活因子(如GAL4等)的DNA结合结构域(DNA-BD)和转录激活域(AD)上,构建成融合表达载体,从表达产物分析两种蛋白质相互作用的系统。
目的 构建DLX4酵母双杂交系统并鉴定其自激活作用。
Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation.
目的:检测抗增殖蛋白在酵母双杂交系统中是否具有转录自激活作用。
Objective: To assay the transcriptional activation effect of prohibitin in yeast two hybrid system.
结论应用酵母双杂交系统,获得了一些候选的NECL1胞内区相互作用蛋白。
Conclusion Using yeast two-hybrid system, we got some alternative proteins which may interact with the cytoplasmic tail of NECL1.
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