...析方法观察80例食管鳞癌中FHIT基因的缺失，并用半定量逆转录聚合酶链反应法(reverse transeriptase-polymerase chain reaction，RT-PCR)分析其中20例食管鳞癌FHIT mRNA表达的变化。
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采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法：应用逆转录-聚合酶链反应(RT - pcr)法检测73例急性白血病患者及23例正常人的WT 1及LRP基因的表达。
Methods: Expressions of WT1 and LRP genes were measured in 73 patients with acute leukemia and 23 normal controls by RT-PCR method.
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
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