方法：运用聚合酶链反应技术(polylnerase chain reaction，PCR)及尿素酶实验(rapid urease test，RUT)，检测246例患者唾液及胃黏膜中的Hp。
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方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法采用微量稀释法检测21种抗菌药物的敏感性，运用聚合酶链反应（PCR）技术检测氨基糖苷类修饰酶基因。
METHODS The dilution test was performed to detect the susceptibility to 21 kinds of antimicrobial agents and genotypes of AMEs of 20 E. coli strains were analyzed by polymerase chain reaction(PCR).
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