然后，我们运行所选择的用例并记录软件的执行轨迹 —— 也就是，执行的功能或方法序列。

Then we run the selected use cases and record the execution trace of the software -- i.e., the sequence of functions or methods that is executed.

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此外有些只能在特定的操作系统下运行，大多数软件设置的参数比较多，一般生物学家往往难于掌握，结果同样的序列用不同的软件得到的结果也不同。

Different software use different algorithms and emphasis, Some only can be used in certain operating system. in addition, the same sequence use different parameters will lead to different results.

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