...1,在阳离子脂质体的辅助下转染MCL-1的反义寡核苷酸利用逆转录聚合酶链式反应(Reverse transcription polymerase chain reaction,RT-PCR)的方法观察转染前后MCL-1基因表达情况;免疫组化及Western Blot法检测肿瘤细胞转染前后M..
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...基因组是单链环状DNA(ssDNA),需先将RNA反转录成cDNA,再以此为模板进行扩增，即反转录聚合酶链式反应（Reverse transcription-PCR,RT-PCR），该技术现已是检测病毒的重要手段之一。
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反转录聚合酶链式反应 reverse transcriptase polymerase chain reaction ; RT-PCR ; Reverse-translation polymerase-chain reaction ; Reverse transcription-PCR
逆转录聚合酶链式反应 RT-PCR
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
运用逆转录聚合酶链式反应测定心肌细胞内病毒相关基因的表达。
RT- PCR assays were used to determine expression of CVB_3 - associated genes in cardiomyocytes.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
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