通过某个转化过程形成的,常由于染色体断裂变成异倍体,失去正常细胞特点,而获得无限增殖能力。转化细胞系具有长期培养,倍增时间短,对培养条件和生长因子等要求较低的特点,适于大规模工业化生产。在生物制药中,可通过改造宿主细胞特性,如延长细胞周期.提高工程细胞原始表达水平等来提高药物的产量。
同时,转化细胞系dna、RNA和蛋白质出现最大合成速度的时间均相应地延迟。
Meanwhile, the appearance of the maximum synthesis rate for DNA, RNA and protein in transformed cell line exhibited was postponed compared with control cell line.
目的研究EZH2蛋白在食管上皮永生化细胞系(SHEE)和恶性转化细胞系(SHEEC)中的表达。
Objective To investigate the expressions of EZH2 protein in the human immortalized esophageal epithelial cell line (SHEE) and the malignant transformation cell line (SHEEC).
在细胞生物学中用嘌罗霉素来选择用载体转化能表达嘌呤霉素- N -乙酰基转移酶类的哺乳动物细胞系。
Puromycin is used in cell biology to select mammalian cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase.
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