全骨髓法培养大鼠骨髓间充质干细胞及其生物学特性-临床医学论文-论文联盟 关键词】 间充质干细胞;骨髓;贴壁法分离培养 [gap=1427]Key words: mesenchymal stem cells; bone marrow; attachment culture
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方法贴壁法分离培养外周血单核细胞并进行鉴定。
Methods the monocytes were separated by the anchoring technique and identified.
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
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