质粒载体是在天然质粒的基础上为适应实验室操作而进行人工构建的质粒。与天然质粒相比，质粒载体通常带有一个或一个以上的选择性标记基因（如抗生素抗性基因）和一个人工合成的含有多个限制性内切酶识别位点的多克隆位点序列，并去掉了大部分非必需序列，使分子量尽可能减少，以便于基因工程操作。
质粒载体 [zhì lì zǎi tǐ]
网络释义专业释义
一、质粒载体（plasimid vectors） 2、质粒的大小 差异很大，最小的只有1kb,只能编码中等大小的2-3种蛋 白质分子，最大的达到200kb。
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plasmid vector - 引用次数：36
The screened aptamters are purified and connected to pGEM-T plasmid vector , and 24 clones of random selection are sequenced after screening by the blue and white.
将筛选得到的适配子回收纯化后连接pGEM-T质粒载体,经蓝白筛选后,随机挑选24个克隆子进行序列测定。
参考来源 - 慢性粒细胞白血病K562细胞适配子的筛选、鉴定与结构分析 -
plasmid vector - 引用次数：6
Conclusion The plasmid vector pZCY11 made it more convenient for the study on functions and the expressions of target gene in M. xanthus.
结论构建的质粒载体pZCY11不仅能够对目的基因进行功能的分析,而且能够同时通过报告基因分析基因的表达情况,可简化粘球菌中基因功能及表达情况的研究。
参考来源 - 粘球菌基因功能和表达情况分析质粒pZCY11的构建及其应用
·2,447,543篇论文数据，部分数据来源于NoteExpress
双语例句原声例句
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目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
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目的：构建基质金属蛋白酶9靶向的RNA干扰质粒载体，观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
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这些载体具有不同的特征，包括质粒、粘粒和酵母人工染色体。
These vectors can have different features (such as the size of DNA-insert they can accommodate) and include plasmids, cosmids, and yeast artificial chromosomes (YACs).
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The sources are: one,the plasmid vector that we've picked, and the second is these genes that for some reason we're interested in.
第一个是我们选择的质粒载体,第二个是我们感兴趣的基因
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The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.
接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配
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Then we're going to take these plasmid vectors and we're going to somehow put them in contact with cells in such a way that the cells ingest the DNA and they use it.
然后要使这些质粒载体,以一种特别的方式与细胞接触,让细胞吞入质粒载体并使之表达
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