组织含褐藻酸盐裂解酶 alginate lyase
分段盐析的提纯倍数为(以粗酶提取液为参照)褐藻酸酶13.3 ,琼脂酶8.7和纤维素酶10.9。
The number of purification times of algalase was 13.3 (crude extraction liquid as control ), 8.7 for agaraseand 10.9 for cellulase.
采用上述酶降解褐藻酸钠得到的产物用TLC检测得知含有单糖醛酸,用ESI-TOF-MS检测得知含有二糖至八糖的混合褐藻酸寡糖。
Sodium alginate were degradated by the enzyme. It can be proved by TLC and ESI-TOF-MS that mono-uronic acid and DP of Alginic acid oligosaccharides between two to eight were contained.
应用推荐