方法：应用免疫组化和形态计量方法，检测80例骨肉瘤TAF表达和肿瘤微血管密度。
Methods The expression of TAF and microvascular density count in 80 osteosarcomas were investigated by immunohistochemical staining and morphometric technique.
方法应用免疫组化和形态计量方法，检测80例骨肉瘤VEGF表达、肿瘤微血管密度（MVD），以及OS-732血管生成相关因子的表达。
Methods The expression of VEGF and intratumoral microvascular density(MVD) in 80 osteosarcomas and osteosarcoma cell OS-732 was detected by immunohistochemical staining.
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