目的构建天花粉蛋白(TCS)突变体基因，并进行表达及纯化。
Objective To construct and express a trichosanthin (TCS) gene mutant and purify the expressed product.
结论：成功地克隆、表达及纯化NBEAL1蛋白，NBEAL1蛋白在人脑胶质瘤组织中的表达与其恶性程度呈负相关。
Conclusion: the NBEAL1 protein has been successfully cloned, expressed and purified. NBEAL1 protein expression in glioma tissues is negatively associated with the pathological grades of glioma.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
应用推荐