正常对照组行假手术后，每日皮下注射等量生理盐水。
The control group were treated with sham operation and placebo.
麻醉后，去卵巢组大鼠切除双侧卵巢，假手术组仅行开腹术。
After anesthesia, rats in the ovariectomized group were treated with bilateral ovariectomy, and those in the sham-operated group were only given laparotomy.
假手术组仅进行右侧颈总动脉分离术而不作结扎及缺氧处理。PVL模型组大鼠行右侧颈总动脉结扎后吸入含6％氧气和94％氮气的氧氮混合气体，处理4小时，建立新生大鼠PVL模型。
Rats in the PVL model group were subjected to the ligation of right carotid artery, and then they were kept in a box with 6% oxygen and 94% nitrogen for 4 hours to establish the PVL animal model.
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