然后,采用密度梯度离心法自成年健康自愿者外周血分离单个核细胞(peripheral blood mononuclear cells,PBMC),并采用尼龙毛柱分离CD3~+T淋巴细胞。
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然后,采用密度梯度离心法自成年健康自愿者外周血分离单个核细胞(peripheral blood mononuclear cells,PBMC),并采用尼龙毛柱分离CD3~+T淋巴细胞。
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外周血分离单个核细胞 PBMC ; peripheral blood mononuclear cells
分离脐血单个核细胞 MNCS ; mononuclear cells
法分离脐血单个核细胞 mononuclear cells
同时由于脐血库液氮保存设备费用昂贵，需要预先分离脐血单个核细胞以减少样本冻存体积，以满足建库实际需求。
Cord blood separation and volume reduction is first needed to meet THD practical bank requirement for the high expense of liquid nitrogen preservation equipment.
目的：探索一种简便、高效的脐血单个核细胞分离方法。
Objective: To explore an convenient and high-performance isolation method of mononuclear cells derived from human cord blood.
无菌条件下取正常足月剖宫产胎儿的脐带血，经肝素抗凝，用淋巴细胞分离液分离脐血单个核细胞。
The specimens were obtained sterilely with preservative-free heparin, and the cord blood mononuclear cell was isolated by lymphocyte separation medium.
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