将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
结果从活化的人白细胞中克隆到IDO基因编码区全长,并构建了IDO EGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
将其与GUS报告基因融合在一起,构建了植物表达载体,并由农杆菌介导法导入水稻品种‘中花11’中。
It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
应用推荐