蛋白样品的制备(Protein sample preparation) 2. 电泳(Electrophoresis) 在收集的蛋白样品中加入适量的SDS-PAGE蛋白上样缓冲液,放置于水漂上于100℃或沸水浴中加热3-5分钟,...
基于25个网页-相关网页
摸索建立了肝组织蛋白样品的制备方法,并初步优化了蛋白质组学研究中的实验条件。
To explore the establishment of protein sample preparation methods for liver tissue and optimize the experimental Parameters.
包括细胞总蛋白样品的制备、微珠上抗原-抗体 反应及微珠洗涤条件、进样方式的 选择与改进。
This part includes preparation of total protein samples from the culture cells, on-bead immuno-binding reaction and wash step, and choice of sampling.
该技术平台的构建涉及到蛋白样品的制备、封闭液的选择、点样条件的选择、抗体的浓度及其杂交时间及数据的处理等多个方面。
This technology platform construction involves protein samples preparation, the choice of sealed liquid, the conditions of printing, concentration and hybrid time of antibody and data processing, etc.
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