癌症000302 关键词： 荧光定量检测；RT-PCR；mdr-1基因；基因表达 [gap=1608]Key words ：Fluorogenic quantitative detecting； RT-PCR； mdr-1 gene； Gene expression
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实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
目的探讨用荧光定量PCR检测不明原因流产组织及血清中巨细胞病毒（CMV）和弓形体（TOX）感染。
Objective: Detect the infection of CMV and TOX in tissue , serum of unexplained abortion by FQ-PCR.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
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