把茎尖的分生组织或包括有此分生组织的茎尖分离进行无菌培养的方法。
二、植物茎尖(stem tip culture)培养： 茎尖培养（shoot tip culture）指对植物顶端的原分生组织和衍生的分生组织的培养。 小到10-100um 的茎尖分生组织，大到几十毫米的茎尖或更大的芽。
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...院文秘网文库 关键词：白杨，茎尖培养，体细胞胚胎发生，悬浮培养，微体繁殖 [gap=1354]Key words: aspen clones, shoot culture, somatic embryogenesis, callus, cell culture ...
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Shoot tip culture、thermotherapy and medicament treatment were the main methods. We studied on the shoot tip culture, rapid propagation and impact factor of virus-free of sweet cherry.
微茎尖培养、热处理和药物处理是目前脱除果树病毒的主要形式。
参考来源 - 甜樱桃茎尖培养快繁体系建立与脱病毒影响因子研究The results showed that shoot tip cultivate can get better effect to get free-CMV seedling,while shoot tip cultivate combined with heat treatment 30 min is superior to tissue culture or shoot tip cultivate to get free-LSV seedling.
对于LSV的脱毒,茎尖培养结合30 min热处理的脱毒效果优于普通组织培养和茎尖培养。
参考来源 - 茎尖培养及热处理技术在百合脱毒中的应用研究Obtaining virus-free plants by means of meristem tip culture was the available measure to deal with the problem.
通过茎尖培养获得脱毒种苗是防治甘薯病毒病害和减缓种质退化的最为有效的措施。
参考来源 - 甘薯茎尖脱毒、快繁技术及其脱毒苗增产机理的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
目的对怀地黄茎尖培养苗进行病毒学鉴定。
Objective To identify if the tobacco mosaio virus remains of plantlets from shoot-tip culture of Rehmannia glutinosa.
为了克服这一缺点，现在经常是将茎尖培养与热处理相结合来使用。
In order to overcome this shortcoming, the tip is now often a combination of training and heat treatment to use.
技术上通常切取茎尖越小，脱毒效果越好，但是茎尖培养成活率变低。
Technical tip is usually the smaller the cut, the effect of virus-free as possible, but low survival rate of shoot tip culture.
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