目的从胚胎大鼠脊髓神经管中分离神经上皮干细胞并诱导其向多巴胺能神经元方向分化。
Objective to isolate neuroepithelial stem cells from the spinal cord neural tube of the embryonic rat and induce them to differentiate into dopaminergic neurons.
目的：观察脑源性神经生长因子（BDNF）和神经营养因子-3（NT-3）单独及联合应用对体外培养胚胎大鼠脊髓神经元存活和生长发育的影响。
Objective:To study the individual and joint effects of BDNF and NT-3on the sur-vival and growth of neurons from the spinal cord of embryonic rat.
方法：胚胎大鼠脊髓神经细胞原代培养，倒置相差显微镜下进行细胞记数和显微测量，观察神经元存活和生长分化的状况。
Methods: Using primary nerve cell culture, we observed the survival and growth of spinal cord neurons with phase-contract microscope.
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