从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
目的：对不同发育阶段的人胚胎原始生殖细胞（PGC）进行定位并比较其体外培养的差异。
Objective: To study the localization and in vitro culture of human embryonic primordial germ cell(PGC) during different stages of embryogenesis.
胚胎干细胞是来源于附植前胚胎的内细胞团或附植后胎儿的原始生殖细胞具有发育全能性的细胞。
Embryonic stem cell is a kind of undifferentiated pluripotent stem cell, which stems from pr Eimplantation ICM or early fetus primordial germ cell.
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