单核晚期小孢子经培养后，具胚状体发生能力的细胞中央液泡消失，积累淀粉，含丰富细胞器。

The central vacuole began to disappear, and cytoplasm with abundant organelle accumulated starch in some of microspores having embryogenic ability on transfer to culture.

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采用组织培养和石蜡切片法，分析多种因素对防风畸形胚状体发生和发育过程的影响及控制。

The effect of various factors on the occurrence and growth of abnormal somatic embryos were studied.

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由于离体培养比较困难，使得荔枝体胚发生和种质离体保存研究进展缓慢。

As there are difficulties in litchi vitro culture, only few studies reported on somatic embryogenesis and germplasm Cryopreservation.

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