Objective To optimize the expression of keratinocyte growth factor-2 (KGF-2) and to evaluate heparin affinity chromatography and ion-exchange chromatography in view of KGF-2 output and purity. Methods Expression of KGF-2 was optimized on growth density and induction time.
目的优化工程菌的表达,比较肝素亲和层析、离子交换层析两种方法的角质细胞生长因子2(keratinocyte growthfactor,KGF-2)的纯化产量及纯度,以获取高效的提纯工艺。
参考来源 - 角质细胞生长因子2的优化表达及纯化研究·2,447,543篇论文数据,部分数据来源于NoteExpress
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