应用聚合酶链反应得到2株蓝绿藻的毒素聚酮合成酶(PKS)基因,并进行基因序列分析。
Gene of polyketide synthase (PKS) from 2 toxin-producing cyanobacteria was prepared by polymerase chain reaction and the gene sequence was analyzed.
方法从Z 10病毒感染的细胞提取总rna,将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定,应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法利用聚合酶链反应(PCR)产物直接测序,对10例血液标本进行了HLA-DPB1、DRB外显子2的序列分析,并将测定结果与基因型核酸序列数据库进行比较。
Methods Sequences of HLA-DPB1, DRB exon2 of 10 samples were analysed by direct sequencing of PCR products. The results of sequencing were compared with their corresponding sequence data.
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